cd31 microbeads (Miltenyi Biotec)
Structured Review
Cd31 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd31/CD31+MicroBeads%2C+mouse/10__1038_slash_s41419___026___09359___9-692-16-18
Average 96 stars, based on 672 article reviews
Images
Related Articles
Incubation:Article Title: Loss of lung mesenchymal identities in culture and a screening approach to identify restorative cues Article Snippet: .. WLCS was incubated with 2μL per 1x10 6 cells of FcR Blocking Reagent (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD326:Magnetic-Bead Antibody (Miltenyi Biotec, 130-061-101, RRID: AB_2832928) for 30 minutes, and Blocking Assay:Article Title: Loss of lung mesenchymal identities in culture and a screening approach to identify restorative cues Article Snippet: .. WLCS was incubated with 2μL per 1x10 6 cells of FcR Blocking Reagent (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD326:Magnetic-Bead Antibody (Miltenyi Biotec, 130-061-101, RRID: AB_2832928) for 30 minutes, and Magnetic Cell Separation:Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema Article Snippet: .. Cells were MACS sorted by labelling with biotin-conjugated antibodies against CD45 (#130-110-657, Miltenyi Biotec), Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), other:Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition. Article Snippet: Frozen 4% PFA fixed OCT (Fisher Scientific, Hampton, NH) sections were mounted with Fluoroshield Mounting Media containing DAPI (Vector Laboratories). Staining:Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1 Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and Isolation:Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1 Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and Fluorescence:Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1 Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and FACS:Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1 Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair. Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), Recombinant:Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), Single Cell:Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair. Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), Purification:Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair. Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), RNA Sequencing:Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair. Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), |
