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cd31 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec cd31 microbeads
    Cd31 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31/CD31+MicroBeads%2C+mouse/10__1038_slash_s41419___026___09359___9-692-16-18
    Average 96 stars, based on 672 article reviews
    cd31 microbeads - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Loss of lung mesenchymal identities in culture and a screening approach to identify restorative cues
    Article Snippet: .. WLCS was incubated with 2μL per 1x10 6 cells of FcR Blocking Reagent (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD326:Magnetic-Bead Antibody (Miltenyi Biotec, 130-061-101, RRID: AB_2832928) for 30 minutes, and CD31:Magnetic-Bead Antibody (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD45:Magnetic-Bead Antibody (Miltenyi Biotec, 130-045-801, RRID: AB_2783001) for 15 minutes, diluted in 0.5% BSA AutoMACS Rinsing Solution at 4°C in the dark, per manufacturer instructions. .. Using an LD Column (Miltenyi Biotec, 130-042-901), the suspension was enriched for lung mesenchyme, per manufacturer recommendations.

    Blocking Assay:

    Article Title: Loss of lung mesenchymal identities in culture and a screening approach to identify restorative cues
    Article Snippet: .. WLCS was incubated with 2μL per 1x10 6 cells of FcR Blocking Reagent (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD326:Magnetic-Bead Antibody (Miltenyi Biotec, 130-061-101, RRID: AB_2832928) for 30 minutes, and CD31:Magnetic-Bead Antibody (Miltenyi Biotec, 130-091-935, RRID: AB_3699142) and CD45:Magnetic-Bead Antibody (Miltenyi Biotec, 130-045-801, RRID: AB_2783001) for 15 minutes, diluted in 0.5% BSA AutoMACS Rinsing Solution at 4°C in the dark, per manufacturer instructions. .. Using an LD Column (Miltenyi Biotec, 130-042-901), the suspension was enriched for lung mesenchyme, per manufacturer recommendations.

    Magnetic Cell Separation:

    Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema
    Article Snippet: .. Cells were MACS sorted by labelling with biotin-conjugated antibodies against CD45 (#130-110-657, Miltenyi Biotec), CD31 (#130-111-353, Miltenyi Biotec), CD326 (#130-117-751, Miltenyi Biotec), and CD140a (#130-101-502, Miltenyi Biotec) for 15 minutes at 4°C. ..

    Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue
    Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or isotype controls (all from Miltenyi Biotec: Isotype FITC recombinant human IgG1, clone REA293, Cat. No.: 130-118-354; Isotype VioBlue recombinant human IgG1, REA293, Cat. No.: 130-117-362; Isotype APC recombinant human IgG1, REA293, Cat. No.: 130-120-709). ..

    other:

    Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition.
    Article Snippet: Frozen 4% PFA fixed OCT (Fisher Scientific, Hampton, NH) sections were mounted with Fluoroshield Mounting Media containing DAPI (Vector Laboratories).

    Staining:

    Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1
    Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and CD31 (PECAM-1; Miltenyi Biotec, 1:50, Cat# 130-110-807) antibodies, and double-positive populations were isolated by fluorescence-activated cell sorting (FACS). .. Sorted cells were subsequently cultured using the STEMdiffTM Endothelial Expansion Kit (STEMCELL Technologies, Cat# 100-1218).

    Isolation:

    Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1
    Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and CD31 (PECAM-1; Miltenyi Biotec, 1:50, Cat# 130-110-807) antibodies, and double-positive populations were isolated by fluorescence-activated cell sorting (FACS). .. Sorted cells were subsequently cultured using the STEMdiffTM Endothelial Expansion Kit (STEMCELL Technologies, Cat# 100-1218).

    Fluorescence:

    Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1
    Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and CD31 (PECAM-1; Miltenyi Biotec, 1:50, Cat# 130-110-807) antibodies, and double-positive populations were isolated by fluorescence-activated cell sorting (FACS). .. Sorted cells were subsequently cultured using the STEMdiffTM Endothelial Expansion Kit (STEMCELL Technologies, Cat# 100-1218).

    FACS:

    Article Title: Impaired SOX17 Expression Causes Endothelial Dysfunction and Pulmonary Arterial Hypertension by Insufficient Suppression of RUNX1
    Article Snippet: In the first method, iPSCs were differentiated into ECs using the STEMdiffTM Endothelial Differentiation Kit (STEMCELL Technologies, Cat# 08005) according to the manufacturer’s instructions. .. Following differentiation, cells were stained with CD144 (VE-cadherin; Miltenyi Biotec, 1:50, Cat# 130-123-688) and CD31 (PECAM-1; Miltenyi Biotec, 1:50, Cat# 130-110-807) antibodies, and double-positive populations were isolated by fluorescence-activated cell sorting (FACS). .. Sorted cells were subsequently cultured using the STEMdiffTM Endothelial Expansion Kit (STEMCELL Technologies, Cat# 100-1218).

    Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition
    Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off of LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany/Auburn, CA). .. All human lung tissue samples were de-identified and determined by the National Jewish Health and University of Colorado Anschutz Medical Campus Institutional Review Boards (IRBs) to constitute non–human subjects research; therefore, informed consent and additional IRB approval for this study were not required.

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Recombinant:

    Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue
    Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or isotype controls (all from Miltenyi Biotec: Isotype FITC recombinant human IgG1, clone REA293, Cat. No.: 130-118-354; Isotype VioBlue recombinant human IgG1, REA293, Cat. No.: 130-117-362; Isotype APC recombinant human IgG1, REA293, Cat. No.: 130-120-709). ..

    Single Cell:

    Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition
    Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off of LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany/Auburn, CA). .. All human lung tissue samples were de-identified and determined by the National Jewish Health and University of Colorado Anschutz Medical Campus Institutional Review Boards (IRBs) to constitute non–human subjects research; therefore, informed consent and additional IRB approval for this study were not required.

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Purification:

    Article Title: Conditional BCL-2 Expression in Fibroblasts Promotes Persistent Pulmonary Fibrosis which is Reversible by Therapeutic BCL-2 Inhibition
    Article Snippet: Images were acquired on a Zeiss Axioplan 2 epi-fluorescence microscope and analyzed with Axiovision software (Zeiss, Jena, Germany). .. Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off of LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany/Auburn, CA). .. All human lung tissue samples were de-identified and determined by the National Jewish Health and University of Colorado Anschutz Medical Campus Institutional Review Boards (IRBs) to constitute non–human subjects research; therefore, informed consent and additional IRB approval for this study were not required.

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    RNA Sequencing:

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).



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    Image Search Results


    ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Journal: Science Advances

    Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis

    doi: 10.1126/sciadv.adw6624

    Figure Lengend Snippet: ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Article Snippet: Primary antibodies used to specifically detect the proteins of interest were as follows: SIK2 (6919s, CST), GAPDH (G9545, Sigma-Aldrich), α-SMA (A5228, Sigma-Aldrich), Collagen I (AB765P, Sigma-Aldrich), Collagen III (AB7778, Abcam), CD31 (3528s, CST), Phospho-NF-κB P65 (Ser 536 ) (3033s, CST), NF-κB P65 (8242s, CST), Phospho-P38 MAPK (Thr 180 /Tyr 182 ) (4511s, CST), P38 MAPK (8690s, CST), α-Tubulin (66031-1, Proteintech), Histone H3 (68345-1, Proteintech), NOS2 (13120s, CST), FLAG-Tag (14793s, CST), HA-Tag (3724s, CST), TRAF1 (4710s, CST), TRAF2 (4724s, CST), TRAF4 (18527s, CST), TRAF6 (67591s, CST), His-Tag (12698s, CST), and Myc-Tag (2276s, CST).

    Techniques: Analysis, Staining, Immunostaining, Tissue